In recent years the importance of molecular nutrition as a potential determinant of health and diseases has been highlighted and endorsed as a result of clinical research in the growing number of studies . The focus of the studies has been centred on the interaction between nutrition and gene expression in impacting health outcomes. In particular, the ability of micronutrients to regulate the final expression of genes in our body via the modulation of transcription and translation is now being recognized.
MicroRNA and mRNA
miRNA are an abundant class of short non-coding RNA in our cells, which are involved in post-transcriptional regulation of gene expression. Due to their minuscule micro size, each miRNA may target hundreds of messenger RNA (mRNA). Likewise, each mRNA can be targeted by multiple miRNA, allowing complex and multifaceted regulatory networks. It has been estimated that miRNA have a post-transcriptional regulatory influence on more than half of all human mRNA. As such, they are believed to participate in almost all of our cellular processes.
Modulation of microRNA (miRNA) by nutrients is one pathway by which nutrition may stimulate and activate gene expression. miRNA are able to indirectly influence gene expression potential at the transcriptional level via modulation of the function of components of the epigenetic machinery (DNA methylation and histone modifications). Therefore, the role that nutritional components, in particular, vitamins and minerals, play in the modulation of miRNA profiles, and consequently health and disease, is increasingly being investigated.
According to a landmark study published in the Cambridge University Press, there are several known associations between miRNA and vitamins and minerals like vitamin D, E, zinc and selenium.
Vitamin D is a fat-soluble vitamin that can be consumed in the diet, or is synthesised by the skin in response to sunlight. In addition to its traditional role in maintaining bone health, epidemiological evidence has now identified a potential role for vitamin D deficiency in a range of conditions including diabetes, autoimmune disease and some cancers. Vitamin D supplementation is now being investigated in the treatment and prevention of these diseases, with a particular focus on its potential as a cancer chemo preventative.
Regulation of mRNA levels via miRNA signalling is now becoming recognised as a potential additional mechanism for action for vitamin D, and consequences of these interactions are also being investigated.
Although the evidence generated in cell studies suggests a role for miRNA and vitamin D in modulating disease-related processes, these studies often used “greater than normal “doses. Limited studies involving correlation between serum levels of vitamin D and miRNA expression in human subjects have also been conducted.
Vitamin E belongs to a fat-soluble group of vitamins that includes several vitamers belonging to tocopherols and tocotrienols.
The impact of dietary vitamin E on miRNA expression has been investigated in a rodent model of vitamin E deficiency. The studies suggested that there are increased expression of genes related to lipid metabolism and resulting in a reduction of plasma cholesterol levels.
Selenium is an essential nutrient found in Brazil nuts, shellfish, chicken, organ meats, game meat and beef. It has structural and enzymic biological roles, pro-apoptotic and DNA repair properties and is an important cofactor in the production and activity of antioxidant enzymes. Selenium is vital for human health and deficiencies have been linked to various diseases including cancers, autoimmune disease and cardiovascular diseases.
Association studies have suggested links between low selenium status, cognitive decline, immune disorders and increased mortality. High selenium levels appear to be beneficial in some cancer types, but the results of supplementation intervention trials have been mixed, suggesting that supplementation is only beneficial if dietary intake is inadequate
miRNA modulation is a potential mechanism of action for selenium. Treatment of human prostate cancer cells with selenite, a natural form of selenium has been shown to induce an up-regulation of p53 expression suggesting that selenium may play role in the modulation of the cell cycle in the induction of cancer. Upon activation, p53 induces the expression of a variety of gene products, which cause either a prolonged cell-cycle arrest in G1, thereby preventing proliferation of damaged cells, or apoptosis, thereby removing damaged cells from our body.
Nutritional Modulation of Epigenetic Traits
Diet is one of the most important modifiable determinants of risk in many diseases, with the phenotypic outcomes of adverse dietary habits accumulating over a lifetime. Chronic diseases or diseases with age-related onset, such as cancer, CVD and diabetes, are particularly well recognised as having associated life-style risk factors, in particular dietary components( 76 , 77 ). Diet also plays an additional ongoing role in disease, as patients suffering from chronic diseases may have impaired nutritional status as a consequence of disease( 78 ). Roles for both dietary factors and miRNA have been identified in the functioning and modification of multiple disease-related pathways including DNA methylation, inflammation, cell-cycle repair, apoptosis and DNA repair. This has led to expanding investigations into the relationships between miRNA signatures, dietary habits and disease risk.
Can Caviarlieri, a Potent Food Supplement activate mRNA, trigger Protein Synthesis and help increase gene expression? The Findings.
Revolutionary Scientific Discovery for Caviarlieri – Swiss Caviar Cell Therapy Supplement Activates Gene Expression through Cellular Nutrition.
Initial in- vivo research studies on Caviarlieri at the molecular level have proven that this caviar supplement is effective in the gene expression of genes related to anti-aging, inflammation (reduction in the expression of Cox2 gene), type 1 collagen and energy (GABPB1 gene).
By providing nutrition at the cellular level, Caviarlieri can trigger protein synthesis and prompt our bodies to produce proteins through active gene induction and thereby stimulating and accelerating cellular renewal and repair against damaged cells.
Mirroring the results of the Caviarlieri studies which are published in peer reviewed journals, the current genetic expression studies (mRNA) on Caviarlieri also show that this caviar supplement can potentially help to reduce and control inflammation and delay the onset and progression of degenerative diseases. Taking Caviarlieri supplements over time can therefore activate the body’s own system in cellular renewal and repair against cellular damage through genetic expression.
Dr. Sotiris Nikolopoulos, Founder and Director of the Center for Molecular Analysis & Research S.A., responsible for the in vivo studies on Caviarlieri on genetic expression (mRNA) says that “Caviarlieri shows amazing anti-aging and anti-inflammatory results for patients from ages 18 to 83 years.”
As a research scientist specializing in molecular analysis, Dr. Sotiris Nikolopoulos was initially very sceptical when he was informed about the outstanding benefits of this Caviar Supplement by the Chief of internal medicine at the Metropolitan Hospital Athens, Greece, Dr. Michael Papacharalambous MD.
Dr. Nikolopoulos then decided to embark on a research study of his own on a group of patients from 18-83 years old to ascertain if this Swiss caviar cell therapy product is able to deliver consistent results in terms of genetic expression for the following genes across different age groups –the anti-aging gene, the type 1 collagen gene, GABPB1 energy gene and the Cox2 gene. He was overwhelmed and astonished with the findings in particular the outstanding and consistent outcomes.
Having discovered the above he then undertook to assess the results of the clinical subjects of the biomarkers and blood test measurements at a molecular level over a period of 2 months. Regardless of their age, he found that all clinical subjects showed outstanding improvements in energy levels, type 1 collagen, and a significant reduction in inflammation at the genetic and molecular level. This confirmed that Caviarlieri can potentially trigger epigenetics changes by providing nutrition at the cellular level and providing benefits to those who consume it at a molecular, cellular and genetic level .This is unprecedented and is unheard of any other food supplement.
The research for Caviarlieri on genetic expression is still ongoing. Sotiris Nikolopoulos intends to publish his findings soon.
Caviarlieri is Backed by Science
Prior to the recent studies on mRNA and genetic expression, Caviarlieri already has several Peer Reviewed Scientific Studies published in PubMed and PMC Europe. The benefit claims are therefore evidence based, like significant increase in energy levels, mood elevation, reduction in joint pain, enhancement of sleep quality, improving brain health, strong anti-inflammatory properties and many others. It is important to remember that healing begins at the cellular level. These studies demonstrate Caviarlieri’s effective therapeutic outcomes and its ability to modulate and modify disease-related processes.
Caviarlieri is not a drug but a potent pharmaceutical grade food supplement which can potentially help to delay the onset and progression of degenerative diseases. The list of studies are as follows:
1. Acta Bio Medical/ Official Journal of the Society of Medicine and Natural Sciences of Parma Vol 82 – N2/2012
Title of the report: Improving Sperm Quality and Spermatogenesis through a Bioactive Marine Compound: an experimental study
2. Journal of Biological Regulators and Homeostatic Agents ISSN – 0393-974X/2012
Title of the report: Effective Properties of a Sturgeon-Based Bioactive Compound on Stress-Induced Hippocampal Degeneration and on In Vitro Neurogenesis
3. Acta Bio Medical / Official Journal of the Society of Medicine and Natural Sciences of Parma Vol 84-N1/2013
Title of the report: Testing a novel bioactive marine nutraceutical on osteoarthritis patients
4. Acta Bio Medical / Official Journal of the Society of Medicine and Natural Sciences of Parma Vol 82 – N2/2012
Title of the report: A Sturgeon-derived Bioactive Compound beneficially modulates nuclear receptors controlling metabolic functions in patients with Metabolic Syndrome
5.Rejuvenation Research Volume 15 Number 2, April 2012
Title of the report: Beneficial Modulation from a High-Purity Caviar-Derived Homogenate on Chronological Skin Aging”
6. Journal of Biological Regulators and Homeostatic Agents ISSN – 0393-974X/2012
Title of the report: Beneficial Effect of a Sturgeon-Based Bioactive Compound on Gene Expression of Tumour Necrosis Factor-alpha Matrix Metalloproteinases and Type-10 Collagen in Human Chondrocytes
7. Journal of Clinical and Diagnostics Research V 8(11) November 2014
Title of the report: A Double- Blind, RCT Testing Beneficial Modulation of Bdnf in Middle-Aged, Life Style-Stressed Subjects : A Clue to Brain Protection
8. .Rejuvenation Research Volume 17 Number 2, April 2012 (Europe PMC)
Title of the report: Protective Effect of a Fish Egg Homogenate Marine Compound on Arterial Ultrastructure in Spontaneous Hypertensive Rats
Why is Caviarlieri a Safe and Potent Option?
Caviarlieri is not a drug but an oral food supplement approved by the Swiss Public Health Authority. Unlike drugs or medications, there are no side effects for long term use. Caviarlieri is also certified by the German Sport University, Cologne as being free of steroids, hormones and stimulants which makes it safe for long-term use.
Manufactured using the Swiss Cold Process Extraction Technology, Cellularix, Caviarlieri comes in a soft gel form and is made of peptides, encapsulating the Caviar bioactive nutrients and marine peptides for optimal absorption. It is important to remember that not everything we consume can be absorbed by the body.
By providing essential nutrition at the cellular level, Caviarlieri triggers protein synthesis, genetic expression (mRNA) and thereby stimulates our own body system to heal, repair and renew our cells at an accelerated rate against damaged cells. Unlike drugs, it will take a 3-6 months regime for the cellular healing and repair to generate maximum benefits and results.
Caviarlieri is available in the leading Swiss Medical Centres and Luxury Spas as well as Pharmacies throughout Switzerland.